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Yam in Vitro Gene Banking

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This manual describes the yam in vitro genebanking process set up by IITA.

Within the last 3 years, this process has been successfully used to duplicate over 1000 accessions of yam from field to in vitro culture. Research is still going on to optimise yam in vitro genebanking, especially at meristeming level. The manual also describes minimum requirements in terms of equipment and consumables for yam in vitro propagation and genebanking.

Compilation by: Dominique Dumet, Abigael Adeyemi, and Omena Ojuederie

Intended use:

Intended users: Genebank managers, supervisors, and staff and trainees

Contents

Introduction

A Explant production
B Germplasm in vitro introduction
C Germplasm in vitro multiplication
D Germplasm gene banking

D1 Transfer to genebank
D2 Germplasm regeneration
D3 Germplasm monitoring
D4 Germplasm inventory

E Germplasm acclimatization
F Basic equipment/items required for yam gene banking
G Media and stock solutions preparation and storage
H General recommendations for laminar flow room, growth room and genebank maintenance and use

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.

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