Seed health diagnosis of bread wheat, durum wheat, triticale and related cereals genetic resources

Contributors to this page: CIMMYT – Wheat, Mexico (Thomas Payne), ICARDA, Syria (Ahmed Amri) with inputs also received from CIMMYT – Maize, Mexico (Suketoshi Taba), USDA – National Small Grains Collection, Aberdeen, Idaho, USA (Harold Bockelmann), CGN, Wageningen, The Netherlands (website) and IPK, Gatersleben, Germany (Helmut Knűpffer).

Recommended procedures to detect important quarantine seed pathogens

Conducting a field inspection (photo: ICARDA)


Xanthomonas translucens pv. undulosa (YDC agar and semi-selective XTS agar).


Tilletia spp. (seed washing, filter test).
Ustilago spp. (seed washing, filter test).


Barley stripe mosaic virus (BSMV), [ELISA and TBIA (tissue-blot immunoassay)].

Testing protocols

Applying the latest laboratory methods, CIMMYT staff insure that seed entering and leaving the center is disease-free (photo: CIMMYT)

Test the accession seed samples before the accession is processed for inclusion in the active genebank collection, or prior to planting in the field to prevent the transfer or establishment of diseases or pests.

Recording information during seed health diagnosis

The following information should be recorded for each step:

Other information

Click here for more detailed outputs from the page on the safe transfer of germplasm on this website.

See also the list of pests and diseases for this crop listed in the crop regeneration guidelines.

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.