Field bank (regeneration) guidelines for banana

Contributors to this page: Bioversity International, France (Nicolas Roux), Bioversity International, Ethiopia (Michael Bolton, Alexandra Jorge); CIRAD,  France (Jean-Pierre Horry, Tomekpe Kodjo); IITA, Nigeria (Dominique Dumet).

Field bank for banana

When are field banks used

Banana field collection (photo: Bioversity)

Many important varieties of field, horticultural and forestry species, including banana, are either difficult or impossible to conserve as seeds (i.e. no seeds are formed or if formed, the seeds are recalcitrant) or to reproduce vegetatively. Hence they can be conserved as growing plants in field genebanks or in vitro (in tissue culture or cryo banks).

Banana stored in field genebanks have a lower risk of loosing genetic integrity (due to genetic drift) if the mother plants are maintained for many years and are readily available for study and use. However, bananas maintained in field genebanks are considerably more exposed to physical risks (climate, diseases, pests) and costs are higher for storage (labour, inputs and space) than for in vitro genebanks. This balance must be considered before taking the decision to establish a field genebank for banana, if other options are also possible.

Advantages of field genebanks

Disadvantages of field genebanks

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Regeneration guidelines for banana

Note: These guidelines refer specifically to the regeneration of banana in field collections and include information on the establishment of a new field collection.
For regeneration or rejuvenation of banana in in vitro collections click here.

The information on this page was extracted from:
Tomekpe K. and Fondi E. 2008. Regeneration guidelines: banana. In: Dulloo M.E., Thormann I., Jorge M.A. and Hanson J., editors. Crop specific regeneration guidelines [CD-ROM]. CGIAR System-wide Genetic Resource Programme, Rome, Italy. 9 pp.

Before reading the regeneration details for this crop, please read the general introduction that gives the general guidelines to follow, by clicking here.


Banana and plantain (Musa spp. L.) are giant monocotyledonous perennial herbs that thrive in the humid and subhumid tropics of low- and mid-altitude areas. They originate primarily from South-East Asia, with secondary centres of diversity in West and Central Africa (Plantain subgroup) and the East African highlands (Lujugira subgroup). They belong to the genus Musa, which comprises more than 1000 varieties in four sections: Australimusa, Callimusa, Rhodochlamys and Eumusa (Simmonds and Shepherd 1955). Most cultivated Musa belong to the Eumusa section and produce fruits that are a major commodity in international trade but are even more important as starchy staples in local food economies of many developing countries.
Most cultivars are derived from two species: Musa acuminata Colla (A genome) and Musa balbisiana Colla (B genome). Most edible banana are triploids (2n = 3x = 33), although there are a few diploid cultivars and even fewer tetraploid cultivars.
Bananas and plantains are also classified according to use (dessert, cooking, beer or processing/fibre) or mode of preparation of the fruit. Sweet bananas are eaten fresh or unprocessed but most bananas, and particularly plantains with orange pulp, are cooked. Cultivated bananas and plantains are parthenocarpic and are propagated vegetatively.
They are conserved in field collections as actively growing plants or in vitro as proliferating tissue. Wild relatives (normally non-parthenocarpic) are conserved in the same way. Bananas and plantains consist of an underground organ (corm) that bears roots, suckers or shoots and a pseudostem with leaves. The corm or rhizome is the true stem. Suckers develop initially as swollen buds from lateral meristems at leaf bases on the corm. A sucker has different developmental stages (Stover and Simmonds 1987): peeper, sword sucker and maiden sucker. The recommended stage for regeneration is the sword sucker (a sucker that is 50-150 cm in height with lanceolate leaves) followed by the peeper (a large green bud that has just emerged above ground). Among the followers or daughter plants, a sucker or ratoon is selected to succeed the mother plant. A group of suckers from a single parent is referred to as a stool or mat. A crop cycle is the time between planting and fruit harvest on the same mat. The second harvest from the mat is called the first ratoon crop (Gowen 1995).

Description is an important step in regeneration:
plant at optimal stage for observation (photo: Emmanuel Fondi)

Choice of environment and planting

Climatic conditions

Banana grows well:

However, banana can grow in a much wider range of climates within the tropics, with site selection mainly limited by soil type and rainfall.

Planting season

Planting can be done all year round if there is enough moisture. Planting should be done at the beginning of the rainy season.

Preparation for regeneration

When to regenerate

Regenerate field collections every four years to restore collections, as accumulated diseases and pests reduce plant vigour. Regeneration also allows maintenance (re-alignment) of planting pattern and optimal density since successor plants of banana emerge at variable distances away from the parent stand.

Sword sucker obtained from parent plant for regeneration (photo: Emmanuel Fondi)

Field selection and preparation

Method of regeneration

Both parthenocarpic edible bananas and their wild relatives are regenerated vegetatively. In this way the genetic identity remains unchanged from one cycle to the next. To safeguard the entire biodiversity, it is necessary to regenerate all the accessions.

Planting layout, density and distance

The planting layout of a banana collection should take account of the genomic constitution of the varieties and the use types of the accessions. Make a field plan (electronic and hard copy). See sample plan below.

Divide the site into main blocks separated by 6-m-wide tracks. Blocks should correspond to the genomic group or the main subgroup, e.g. plantain. Assign block names and then:

Parent plant with suckers to be used for regeneration. (photo: Emmanuel Fondi)

Source of planting material

Selection of planting material

The following diseases must be avoided: Moko disease due to Ralstonia solanacearum Smith, phylotype II, Xanthomonas wilt (bacteria wilt) caused by Xanthomonas vasicola pv. musacearum, Panama disease (Race 1 and 2) and other vegetative compatibility groups (VGCs) of Fusarium oxysporum f. sp. cubense, Banana bunchy top virus (BBTV), Banana bract mosaic virus (BBrMV).

Other pests and diseases, especially nematodes, weevils and other viral and bacterial diseases, should be absent or have a low prevalence in the field from which planting material is extracted. Monitor by regular field inspections.

Preparing planting material and method

Paring of sucker to eliminate soil-born pests and diseases (photo: Emmanuel Fondi)

It is indicated to plant the suckers at once after their pulling up and treatment and if it is not possible, one or a few days afterwards suckers can however be stored for several weeks in a shaded dry area until planting is completed.


Metallic plate to identify accession (photo: Emmanuel Fondi)

Crop management


Fertilizer practices vary widely according to climate, cultivar, yield level, soil fertility and production system. Before planting, take a composite soil sample from each block or from each soil type change. Analyse the sample to determine soil pH and micronutrient levels. This should provide a reliable recommendation for pre-planting application of lime (dolomitic or calcitic), K and P. The normal pH range is 5.8 to 6.5. Below this, lime application is required. Top dressing with N and K is recommended according to the expected yield level and results of soil analysis.

Weed management

Weeds can be manually or chemically controlled.


Mulch with organic matter to reduce evapotranspiration and to increase the organic matter content of the soil. Elephant grass (Pennisetum purpureum Schumach.) is a popular mulch in Central Africa, particularly for plantains and diploid bananas. It is rich in potassium and improves soil fertility.

Pruning and de-suckering


Irrigation is required during the dry season to keep the plants vigorous.

Common pests and diseases

Major pests of banana include nematodes, banana weevil borer, tomato semi-looper (Chrysodeixis acuta) and thrips.
Major diseases include Panama wilt (Fusarium oxysporum), bacterial wilt (Xanthomonas wilt), Erwinia rhizome rot, black and yellow Sigatoka (Mycosphaerella), cucumber mosaic virus (CMV), banana bunchy top virus (BBTV) and banana streak virus (BSV). Do not distribute accessions contaminated with viruses.

Pest and disease control


The regeneration of bananas does not involve seeds. At maturity (when first ripe fruit appears on bunch), description is carried out and the fruits taken for consumption in the case of parthenocarpic edible banana types. For wild types, the entire plant is cut down and placed in the interline space.

Regeneration of wild banana

The regeneration procedures for wild banana should be the same as for edible types.

Monitoring accession identity

Comparisons with passport or morphological data

Accessions are characterized using minimum descriptor forms adapted from‘Descriptors for bananas’ (IPGRI/INIBAP, CIRAD 1996). Typical reference materials are photos and description forms.
Reference sources for comparison include Musalogue (Daniells et al. 2001) and the Musa Germplasm Information System (MGIS) database.
Compare the following major traits in characterization data:

An accession is declared true to type (TTT) if its traits match those of the known reference.
In cases where they do not match with the reference, it is declared either as miss-labelled (ML) or off type (OT). If it is mislabelled, its true identity is sought; whereas if declared off-type, it is destroyed and the right accession re-introduced in to the collection.

Documentation of information during regeneration

The following information should be collected during regeneration:

References and further reading

Daniells J, Jenny C, Karamura D, Tomekpe K. 2001. Musalogue: Diversity in the genus Musa. IPGRI/INIBAP/CTA, Rome, Italy. Available from: (16 MB). Date accessed: 23 March 2010.

IPGRI, INIBAP, CIRAD. 1996. Descriptors for Banana (Musa spp.). IPGRI, Rome, Italy; INIBAP, Montpellier, France; CIRAD, France. 55 pp. Available here.

Lassoudiere A. 2007. Le bananier et sa culture. Editions Quae, Versail es Cedex, France. 383 pp.

Purseglove JW. 1972. Tropical Crops. Monocotyledons. Vol. 2. Longman, London, UK.

Robinson JC, de Villiers EA. 2007. The cultivation of banana. ARC-Institute for Tropical and Subtropical Crops, Nelspruit, South Africa/Du Roi Laboratory, Letsitele, South Africa. 258 pp.

Simmonds, N.W. and Shepherd, K. 1955. The taxonomy and origins of the cultivated bananas. Journal of the Linnean Society (Bot.), 55(359), p. 302-312 Abstract available from: Date accessed: 23 March 2010.

Stover RH, Simmonds NW. 1987. Bananas. Longman Scientific and Technical, New York, USA. 468 pp.

Swennen, R. 1990. Plantain Cultivation under West African Conditions: A Reference Manual. IITA, Ibadan, Nigeria. 24p.


These guidelines have been peer reviewed by Sebastião de Oliveira e Silva, Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical (CNPMF), Brazil; Wayne Hancock, Bioversity International, Ethiopia; and Jeff Daniells, Department of Primary Industries & Fisheries, Queensland, Australia.

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.