Rejuvenation of banana tissue culture

Contributors to this page: Bioversity International, Belgium (Ines Van den Houwe); IITA, Nigeria (Dominique Dumet, Badara Gueye).

Greenhouse regeneration
(photo: Bioversity)

Why regeneration/rejuvenation of stored tissue cultures

Regeneration and rejuvenation of an accession involves the transfer of the accessions to the greenhouse and field in order to check their trueness-to-type (identity and conformity). Rejuvenation is the replacement of the tissue cultures in storage by a set of quality material derived from the verified material. Often both activities are combined.

Using field established plants for harvesting shoot tips to rejuvenate the accession in storage would require re-indexation for viruses, as the plants could have been exposed to viruses.

In order to rejuvenate an accession without having to go through re-indexation of the material, in vitro plants from the accession should first be established in the greenhouse.

It is recommended that this standard procedure involving regeneration and rejuvenation of accessions should be carried out for any accession being maintained continuously in vitro for more than ten subculture cycles (or ten years in MTS):

Regeneration of plants in the greenhouse

Decapitation of suckers

In vitro plants derived from two shoot tips must be sent to the field for verification. If these plants are confirmed true-to-type (after two growing cycles), the remaining shoot-tips from the same sucker can be further multiplied in vitro and replace the old set of replicates in cold storage.

The whole regeneration/rejuvenation cycle takes about 20-22 months.

Field verification and characterization of accessions

References and further reading

Van den Houwe I, Panis B, Arnaud E, Markham R, Swennen R. 2006. The management of banana (Musa spp.) genetic resources at the IPGRI/INIBAP gene bank: The conservation and documentation status. In: Segers H, Desmet P, Baus E, editors. Tropical Biodiversity: Science, Data, Conservation. Proceedings of the 3rd GBIF Science Symposium. Brussels, Belgium, 18-19 April 2005. pp.141-150.

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.