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Health diagnosis of forage legume genetic resources

Contributors to this page: ILRI, Ethiopia (Jean Hanson, Juvy B. Cantrell, Janice Proud); CIAT, Cali, Colombia (Maritza Cuervo); ICARDA, Syria (Siham Asaad, Ahmed Amri, Kenneth Street, Ali Shehadeh, Natalya Rukhkyan); Bioversity International/ILRI, Addis Ababa, Ethiopia (Alexandra Jorge).

A field inspection of grasspea (photo: ICARDA)

Visual virus inspection (photo: ILRI)

List of pests and diseases of quarantine importance

Forage legumes are susceptible to a wide range of pests and diseases. Click here for more detailed and updated information from the page on the safe movement of germplasm, on this website.

Click the forage legume health table for specific species information about health diagnosis methods to detect some of these diseases.

Options for testing procedures

Recommended methods to detect the presence of each pest or disease:

Testing intervals/seasons

Testing before material goes into the genebank or to the field is important to reduce transfer of diseases or pests.

Virus

Fungus

Ascochyta spp., Phoma spp., Botrytis cinerea.

Nematodes

Ditylenchus dipsaci.

Weeds

Orobanche spp. and Cuscuta spp.

Recording information during health diagnosis

The text for this flip book was extracted from: Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowel D, Larinde M. 2006. Manual of seed handling in genebanks. Handbooks for Genebanks No. 8. Bioversity International, Rome, Italy. 147pp.

The following information should be recorded for each health diagnosis step:

References and further reading

Albrechtsen SE. 2006. Testing Methods for Seed-Transmitted Viruses: Principles and Protocols. CABI Publishing, Cambridge, USA. pp. 268.

Nan ZB, Hanson J. 1998. Detection of seed borne fungi in Stylosanthes hamata, S. guianensis and S. scabra. Seed Science and Technology 26:333-345.

Nan ZB, Hanson J, Yeshi MW. 1998. Effects of sulphuric acid and hot water treatments on seed borne fungi and germination of Stylosanthes hamata, S. guianensis and S. scabra. Seed Science and Technology 26:33-43.

Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowel D, Larinde M. 2006. Manual of seed handling in genebanks. Handbooks for Genebanks No. 8. Bioversity International, Rome, Italy. Available in English (1.5 MB),  Spanish (1.4 MB) and French (1.9 MB).

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.

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