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Viability of forage legume genetic resources | Conservation of forage legume genetic resources | Forage legume genetic resources | Crops CGKB | Resources | Genebank Platform

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Viability of forage legume genetic resources


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Contributors to this page: ILRI, Ethiopia (Jean Hanson); ICARDA, Syria (Ahmed Amri, Kenneth Street, Ali Shehadeh, Natalya Rukhkyan); GRCTPL, Australia (Richard Snowball); Bioversity International/ILRI, Addis Ababa, Ethiopia (Alexandra Jorge); CIAT, Cali, Colombia (Daniel Debouck).

Contents:
Viability testing methods to use
Routine monitoring practices

Viability testing

Laboratory method

seed-germinationorginal-(il.jpg

Seed germination tests (photo: ILRI)

Viability test methods are specific for different species. International Seed Testing Association rules should be used when available. Specific information is available for many species (see Species Compendium).

Type of test

or

Number of seeds and replicates

The text for these flipbooks was extracted from: Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowel D and Larinde M. 2006. Manual of seed handling in genebanks. Handbooks for Genebanks No. 8. Bioversity International, Rome, Italy. 147pp.

International Seed Testing Association rules should be used when available.

Pre-treatment

International Seed Testing Association rules should be used when available.

Media

International Seed Testing Association rules should be used when available.

Temperature

International Seed Testing Association rules should be used when available.

Light

International Seed Testing Association rules should be used when available.

Duration of test

International Seed Testing Association rules should be used when available.

The picture above shows abnormal (left) and normal (the last two on the right) legume seedlings. (photo: ILRI)

Others

Monitoring intervals

Legume seeds are usually long-lived. The sampling interval should be spaced so as not to allow seeds to fall below 65% viability but not so frequent as to waste seeds for unnecessary testing:

Recording information during viability testing

The following information must be recorded for each testing step:


Routine monitoring

Describes the recommended monitoring methods to assure minimum viability and quantity of seeds in storage.

Monitoring frequency

Critical quantity

Critical germination level

Recording information during routine monitoring

The following information should be recorded for each step:

References and further reading

Akinola JO, Afolayan RA, Olorunju SAS. 1991. Effects of storage, testa colour and scarification method on seed germination of Desmodium velutinum (Willd.) DC. Seed Science and Technology 19:159-166.

FAO/IPGRI. 1994. Genebank standards. Food and Agriculture Organization of the United Nations, Rome and International Plant Genetic Resources Institute, Rome. Available in English, Spanish, French and Arabic.

International Seed Testing Association [homepage of (ISTA)] [online]. Available from: http://www.seedtest.org/en/home.html. Date accessed: 17 Jan 2011.

ISTA. 2006. Handbook on Seedling Evaluation, 3rd Edition.

Souza FHD, Marcos-Filho J. 1993. Physiological characteristics associated with water imbibition by Calopogonium mucunoides Desv. seeds. Seed Science and Technology 21:561-572.

Thormann I, Metz T, Engels JMM. 2004. The Species Compendium (release 1.0; December 2004). [online] Available from: http://www.bioversityinternational.org/databases/species_compendium_database/index.html. Date accessed: 09 April 2013.

Wang YR, Hanson J. 2008. An improved method for breaking dormancy in seeds of Sesbania seban. Cambridge University Press. Experimental Agriculture. Vol. 44, pp. 185–195. Abstract available from: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1842236. Date accessed: 17 Jan 2011.

Wang YR, Hanson J. 2008. The impact of temperature on seed germination in diverse accessions of 4 wild Vigna species. Proceedings of the XXI International Grasslands Congress, Multifunctional Grasslands in a Changing World. Hohhot, China, June 2008, p. 557.

Wang YR, Hanson J, Wolde YM. 2007. Effect of sulfuric acid pretreatment on breaking hard seed dormancy in diverse accessions of five wild Vigna species. Seed Science and Technology 35:550-559.

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.

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