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Storage of cultivated chickpea and wild relatives genetic resources

Contributors to this page: ICRISAT, Patancheru, India (Hari D Upadhyaya, Shivali Sharma, Cholenahalli L Laxmipathi Gowda, Dintyala Sastry, Sube Singh); NBPGR, New Delhi, India (Shyam Sharma); ICARDA, Aleppo, Syria (Ahmed Amri, Kenneth Street, Natalya Rukhkyan), SARC-RIPP, Piestany, Slovak Republic (Gabriela Antalikova); Institute of Plant Genetic Resources ‘K.Malkov’, Sadovo, Bulgaria (Siyka Stoyanova); Department of Primary Industries, Victoria, Australia (Bob Redden); IPK, Gatersleben, Germany (Andreas Börner).

Contents:
Base collection
Active collection
Storage management

 

 

 

 

 

Base collection

long_term_storage_facility

Long-term storage facility (photo: ICRISAT)

A base collection is a long-term storage. The initial population is used as a bench mark for monitoring changes in genetic diversity over time and after several cycles of regeneration due to genetic drift. It should ensure that an accession’s seed viability remains at acceptable levels for at least 50 years, or at least one cycle of the regeneration of the accession.

When it should be used

Sample specifications

Minimum sample size for storage

A representative sample should be used to avoid genetic drift (Upadhyaya and Laxmipathi, 2009).

Minimum viability for storage

Moisture content

Important to avoid any deterioration pf the stored seed samples.

Container specifications

Seed packaging method

Follow the procedures of the ICRISAT Genebank Manual (Upadhyaya and Laxmipathi, 2009).

Specifications of packaging material

Follow the procedures of the ICRISAT Genebank Manual (Upadhyaya and Laxmipathi, 2009) and FAO/IPGRI Standards, 1994.

Storage specifications

Assigning location codes

Follow the procedures of the ICRISAT Genebank Manual (Upadhyaya and Laxmipathi, 2009).

Storage conditions

Recording information during storage – Base collection

The following information should be recorded for each step:

For large genebanks with active, base and safe duplication strategies, the following alternative is recommended (ICARDA model):

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Active collection

medium_term_storage_facility

Active collection storage facility (photo: ICRISAT)

Active collection at ICARDA (photo: ICARDA)

An active collection consists of accessions that are available for distribution and are accessed frequently. The active collection is intended to preserve germplasm viability in a medium-term storage conditions for at least 20 years or for half of one regeneration cycle in the base collection.

When it should be used

Sample specifications

Minimum sample size for storage

Follow the procedures of the ICRISAT Genebank Manual (Upadhyaya and Laxmipathi, 2009).

Minimum viability for storage

Follow the procedures of the ICRISAT Genebank Manual (Upadhyaya and Laxmipathi, 2009).

Moisture content

To avoid any deterioration of the stored seed samples.

Container specifications

Seed packaging method

Or

Specifications of packaging material

Storage specifications

For easy access and location of the accessions.

Assigning location codes

Storage conditions

Recording information during storage – Active collection

The following information should be recorded for each step:

For large genebanks with active, base and safe duplication strategies, the following alternative is recommended (ICARDA model):

For information on safety duplication, click here.

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Storage management

Storage space arrangement

It is very important to provide easy access to the accessions.

If the genebank’s main function is distibution of germplasm, then adopting a two-step storage system for maintaining active collections and base collections at controlled environments is recommended, otherwise a long-term storage condition should be adopted (Upadhyaya and Laxmipathi, 2009).

System for tracking material/inventory system

Samples must be quickly accessed.

References and further reading

FAO/IPGRI. 1994. Genebank standards. Food and Agriculture Organization of the United Nations, Rome and International Plant Genetic Resources Institute, Rome. Available in English, Spanish, French and Arabic.

GIMS (Genebank Information Management System) – a standalone facility of ICRISAT Genebank. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, A.P. 502 324, India. In: Manual of Genebank Operations and Procedures – Technical Manual no. 6. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, Andhra Pradesh, India: ISBN 92-9066-421-5.

Stoyanova SD. 2001. Ex situ conservation in the Bulgarian genebank. I. Effect of storage. PGR-Newsletter 128:68-76.

Upadhyaya HD, Laxmipathi Gowda CL. 2009. Managing and Enhancing the Use of Germplasm – Strategies and Methodologies. Technical Manual no. 10. Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics. 236 pp.

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The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.

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